By Anthony S. Fauci, R. Ballieux
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Additional info for Antibody Production in Man: In Vitro Synthesis and Clinical Implications
Blood lymphocytes tested under similar conditions did not respond readily. This finding indicated that distinct populations of B cells reside in different lymphoid organs in humans, and that the responsiveness registered in blood lymphocytes might not be representative of the response of all human B cells. A careful and extensive study by Kunori et al. (1978) has revealed different response patterns for human blood and splenic lymphocytes by LPS, and above all, has shown that · with the correct experimental protocol, blood lymphocytes respond well to LPS.
Fauci and Barton F. Haynes 26 I M | EXPECTED RESPONSE OBSERVED RESPONSE CO-CULTURES OF: RESPONDERS CO-CULTURES OF: RESPONDERS RESPONDERS NON-RESPONDERS + + FIGURE 5. Effect of co-culture of non-responder with responder lymphocytes. 01). There is no suppression when responder and allogeneic responder lymphocytes are co-cultured. Data represent the mean (±SEM) of 8 separate experiments. Subsequent experiments were performed to determine which subpopulation(s) of mononuclear cells were responsible for this suppressor phenemenon.
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Antibody Production in Man: In Vitro Synthesis and Clinical Implications by Anthony S. Fauci, R. Ballieux