This choice of displays from the Ciba origin Symposium of 1985 bargains with the significant position of calcium in intracellular techniques. Discusses keep an eye on of intracellular calcium in addition to keep an eye on via intracellular calcium, masking such themes as muscle contraction, metabolic tactics, hormone and transmitter secretion, membrane shipping and permeability, mobile structure and development, and the prospective contribution of calcium gradients to early embryonic improvement. comprises rigorously edited and broad (almost part the booklet) discussions of bankruptcy themes among lively staff within the box on the finish of every bankruptcy.
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Additional resources for Ciba Foundation Symposium 122 - Calcium and the Cell
2. The intramembrane charge moved by various pulses that produce just detectable contraction is constant. Each pair of records give the charge movement current (bottom) and the charge moved ( Q , top) for pulse depolarizations to the membrane potentials indicated in mV next to each pair of records. The vertical dotted lines give the pulse duration required to produce microscopically just-detectable contraction at the same voltage. The intersection of a dotted line with its corresponding Q record gives the charge moved to produce detectable contraction, which was the same for all pulses.
Fibroblasts respond to platelet-derived growth factor (PDGF) with rapid hydrolysis of 46 BERRIDGE PtdInsP, to give D G (Habenicht et a1 1981) and InsP, (Berridge & Irvine 1984). The latter has been shown to release calcium from the intracellular stores in permeabilized Swiss 3T3 cells and thus accounts for the way in which PDGF and vasopressin can increase intracellular calcium levels in these fibroblasts, even in the absence of external calcium (Moolenaar et a1 1984, Hesketh et a1 1985). As in oocytes, the DG/protein kinase C signal pathway activates a Na+/H+ exchanger which extrudes protons in exchange for sodium and so alkalinizes the cytoplasm, which seems to be a prerequisite for DNA synthesis.
Two INOSITOL TRISPHOSPHATE AND CALCIUM MOBILIZATION 41 FIG. 1. Summary of agonist-sensitive phosphoinositide metabolism. 5-bisphosphate(PIP,) to give diacylglyerol (DG) and inositol trisphosphate (Insl,4,5P3). The latter functions by releasing calcium. Stimulated cells also produce Ins1,3,4,5P, but its source is unknown. 4,5P3. 4P3), possibilities which currently are being examined are that InsP, is derived by phosphodiesteratic cleavage of phosphatidylinositol 3,4,5-trisphosphate (PtdIns3,4,5P3).
Ciba Foundation Symposium 122 - Calcium and the Cell