New PDF release: DNA Vaccines: Methods and Protocols

By Monica Rinaldi, Daniela Fioretti, Sandra Iurescia

ISBN-10: 1493904094

ISBN-13: 9781493904099

ISBN-10: 1493904108

ISBN-13: 9781493904105

DNA Vaccines: tools and Protocols, 3rd version explores leading edge methods and applied sciences used to layout, convey, and improve the efficacy of DNA vaccines. that includes purposes which could be of serious price in relocating vaccines from learn to hospital, this specified quantity contains sections on DNA vaccine layout and enhancement, supply platforms, construction, purification, and caliber, in addition to chapters on new vaccine purposes. Written within the hugely profitable Methods in Molecular Biology sequence structure, chapters comprise introductions their respective issues, lists of the required fabrics and reagents, step by step, with ease reproducible laboratory protocols, and pointers on troubleshooting and heading off recognized pitfalls.

Authoritative and functional, DNA Vaccines: equipment and Protocols, 3rd Edition serves the $64000 function of extra documenting the opportunity of the DNA vaccination as a platform expertise for therapy and prevention of human disease.

Show description

Read Online or Download DNA Vaccines: Methods and Protocols PDF

Similar immunology books

Schaum's outline of theory and problems of immunology - download pdf or read online

Tricky attempt Questions? ignored Lectures? no longer sufficient Time? thankfully for you, there is Schaum's Outlines. greater than forty million scholars have depended on Schaum's to aid them reach the study room and on checks. Schaum's is the most important to swifter studying and better grades in each topic. each one define provides the entire crucial direction info in an easy-to-follow, topic-by-topic layout.

Read e-book online Bacterial genomes and infectious diseases PDF

The 1st bacterial genome, Haemophilus influenzae, used to be thoroughly sequenced, annotated, and released in 1995. at the present time, greater than 2 hundred prokaryotic (archaeal and bacterial) genomes were accomplished and over 500 prokaryotic genomes are in va- ous levels finishing touch. Seventeen eukaryotic genomes plus 4 eukaryotic chro- somes were accomplished.

Jessica R. Burdman, Michael F. Powell, Mark J. Newman's Vaccine Design: The Subunit and Adjuvant Approach PDF

Vaccine layout describes medical theories, product improvement recommendations, and effects from preclinical reviews for plenty of of the latest subunit vaccines or parts designed to be used in subunit vaccine formula, reminiscent of adjuvants and vaccine supply structures. The papers contain the main huge choice of vaccine applied sciences and items ever provided in one quantity and gives a useful precis of never-before-published vaccine adjuvants and excipients.

Extra resources for DNA Vaccines: Methods and Protocols

Example text

Orbital shaker, 37 °C. 2 Transformation of E. coli DH5α Cells 1. Competent E. coli DH5α cells. 2. Plasmids: pVAX1-N3-GFP (N3 vector), pVAX1-E1A-N3LAMP (E1A-N3-LAMP), and pVAX1-ISG-GFP-LAMP (ISGLAMP), and pVAX1-Sc-ISG-GFP (Sc-ISG) encoding for the invariant surface glycoprotein (see Note 1). 3. Sterile liquid LB medium (25 g/L). 4. LB-agar plates (35 g/L) supplemented with kanamycin (30 μg/mL). 5. Water bath. 6. Incubator, 37 °C. 7. Orbital shaker, 37 °C. 8. Cryovials. 9. Glycerol for molecular biology (99 %).

After electrophoresis, remove the gel from the electrophoresis chamber and cut it longitudinally between wells two and five. The gel portion containing the molecular weight ladder and the 2 μL sample is then removed from the tray and stained for 20 min in an ethidium bromide solution. Protect the second portion of the gel by placing aluminum foil under it. 16. Put on the UV protection mask, and with a scalpel tip, mark the bands corresponding to the vector pVAX1-N3-GFP and to the different insert targeting sequences on the stained portion of the gel.

Scalpel tip. 14. Transilluminator. 15. QIAquick Gel Extraction Kit (QIAGEN). 16. 80–100 % (v/v) isopropanol. 17. Preheated sterilized MilliQ water at 65 °C (see Note 4). 18. Spectrophotometer. 38 Elisabete Borges Freitas et al. 6 Ligation and Bacterial Cell Transformation Processes 1. Fragment corresponding to the linearized vector pVAX1-N3GFP and the inserts (E1A, LAMP, or Sc). 2. T4 DNA ligase 3 U/μL. 3. 8, 100 mM MgCl2, 100 mM DTT, 10 mM ATP. 4. MilliQ sterilized water. 5. DNA SpeedVac® Concentrator.

Download PDF sample

DNA Vaccines: Methods and Protocols by Monica Rinaldi, Daniela Fioretti, Sandra Iurescia


by Ronald
4.0

Rated 4.78 of 5 – based on 23 votes